Double-disc Electrophoresis of Proteins

Micro-disc electrophoresis of soluble proteins in rabbit blastocysts.

Soluble proteins were studied in the developing rabbit blastocyst by using a micro-disc electrophoresis technique. The tissues of the blastocyst (in later stages separated into the embryoblastic and trophoblastic parts) were analysed, and their protein patterns were compared with those of uterine secretion and blastocyst fluid. A protein pattern with more than 20 protein fractions was found in ...

Disc Electrophoresis. Ii. Method and Application to Human Serum Proteins.

The purpose of this paper is to describe a technique of zone electrophoresis in polyacrylamide gels. The salient characteristics of the technique reside in (1) the controlled variation of the gel pore size for the purpose of increasing the resolution of ions based on dimensional differences and (2) an electrophoretic step for concentrating the sample ions into a narrow starting zone prior to el...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Disc electrophoresis of rat plasma lipoproteins.

The disc electrophoresis of lipoproteins of unfractionated rat plasma is described. The plasma was prestained with Sudan black B and electrophoretically separated at polyacrylamide gel concentrations of 7.5, 5, and 3.75%. At least four lipoprotein components were observed, and an additional 2-3 components in the main gel and 2-5 components in the spacer gel possibly were present. Densitometry o...

Polyacrylamide Disc Electrophoresis of the Proteins of Cerebrospinal Fluid and Brain.

Since one of us (J.N.C.) in 1953 recorded the results of electrophoresis on paper of the proteins of the cerebrospinal fluid and cerebral cyst fluids a number of other supporting media have been employed for this purpose. Some, such as starch, are valuable because of a higher resolving power and a greater ability to separate many of the protein components. The use of the polyacrylamide disc ele...